Researchers studying how genes control animal behavior have had to deal with many uncertainties. the first place, most behaviors are governed by more than one gene, and until recently geneticists had no method for identifying, the multiple genes involved. In addition, even when a single gene is found to control a behavior, researchers in different fields do not necessarily agree that it is a "behavioral gene." Neuroscientists, whose interest in genetic research is to understand the nervous system (which generates behavior), define the term broadly. But ethologists--specialists in animal behavior--are interested in evolution, so they define the term narrowly. They insist that mutations in a behavioral gene must alter a specific normal behavior and not merely make the organism ill, so that the genetically induced behavioral change will provide variation that natural selection can act upon, possibly leading to the evolution of a new species. For example, in the fruit fly, researchers have identified the gene Shaker, mutations in which cause flies to shake violently under anesthesia. Since shaking is not healthy, ethologists do not consider Shaker a behavioral gene. In contrast, ethologists do consider the gene period (per), which controls the fruit fly's circadian (24-hour) rhythm, a behavioral gene because files with mutated per genes are healthy; they simply have different rhythms.

    Anole lizard species that occur together (sympatrically) on certain Caribbean islands occupy different habitats: some live only in the grass, some only on tree trunks, and some only on twigs. These species also differ morphologically: grass dwellers are slender with long tails, tree dwellers are stocky with long legs, twig dwellers are slender but stubby-legged. What is striking about these lizards is not that coexisting species differ in morphology and habitat use (such differences are common among closely related sympatric species), but that the same three types of habitat specialists occur on each of four islands: Puerto Rico, Cuba, Hispaniola, and Jamaica.Moreover, the Puerto Ricantwig species closely resembles the twig species of Cuba, Hispaniola, and Jamaica in morphology, habitat use, and behavior. Likewise, the specialists for other habitats are similar across the islands.

    The presence of similar species on different islands could be variously explained. An ancestral species might have adapted to exploit a particular ecological niche on one island and then traveled over water to colonize other islands. Or this ancestral species might have evolved at a time when the islands were connected, which some of these islands may once have been. After the islands separated, the isolated lizard populations would have become distinct species while also retaining their ancestors' niche adaptations. Both of these scenarios imply that specialization to each niche occurred only once. Alternatively, each specialist could have arisen independently on each of the is lands.

    If each type of specialist evolved just once, then similar specialists on different islands would be closely related. Conversely, if the specialists evolved independently on each island, then a specialist on one island would be more closely related to other types of anoles on the same island-regardless of their ecological niches-than it would be to a similar specialist on a different island.

    Biologists can infer how species are related evolutionarily by comparing DNA sequences for the same genes in different species. Species with similar DNA sequences for these genes are generally more closely related to each other than to species with less-similar DNA sequences. DNA evidence concerning the anoles led researchers to conclude that habitat specialists on one island are not closely related to the same habitat specialists elsewhere, indicating that specialists evolved independently on each island.

    First identified in 1969, komatiites are Earth's oldest known volcanic rocks and contain three times as much magnesium as do most volcanic rocks. This chemical composition suggests that komatiites formed from the hottest lava known ever to have erupted:a high concentration of magnesium changes the physical properties of lava so that unusually high temperatures would be required for the lava to exist as a liquid.

    Komatiites' discovery was surprising in light of then-current geological theories about magmas, molten rock that forms in the Earth's mantle (the layer beneath the crust) and composes volcanic lava eruptions. Prior to 1960, geologists Bowen and Hess disagreed over whether or not the very high temperatures needed to produce magmas rich in magnesium could have existed on Earth. Hess suggested that the presence of water, probably released from minerals decomposing in the Earth's mantle, might have meant that a high-magnesium magma could have existed at a lower temperature. But Bowen showed experimentally that the high temperatures were indeed necessary. By 1960, it was generally accepted that volcanic rocks with such high levels of magnesium could not exist, and thus the discovery of koma tiites changed geologists' assumptions about the characteristics of the Earth's mantle around the time of the formation of komatiites, between 2.5 and 4 billion years ago.