More selective than most chemical pesticides in that they ordinarily destroy only unwanted species, biocontrol. agents (such as insects, fungi, and( viruses) eat, infect, or parasitize targeted plant or animal pests. However, biocontrol agents can negatively affect nontarget species by, for example, competing with them for resources: a biocontrol agent might reduce the benefits conferred by a desirable animal species by consuming a plant on which the animal prefers to lay its eggs. Another example of indirect negative. consequcnces occurred in England when a virus introduced to control rabbits reduced the amount of open ground (because large rabbit populations reduce the ground cover), in turn reducing underground ant nests and triggering the extinction of a blue butterfly that had depended on the nests to shelter its offspring. The paucity of known extinctions or disruptions resulting from indirect interactions may reflect not the infrequency of such mishaps but rather the failure to look for or to detect them: most organisms likely to be adversely affected by indirect interactions are of little or no known commercial value and the events linking a biocontrol agent with an adverse effect are often unclear. Moreover, determining the potential risks of biocontrol agents before they are used is difficult, especially when a nonnative agent is introduced, because, unlike a chemical pesticide, a biocontrol agent may adapt in unpredictable ways. so that it can feed on or otherwise harm new hosts.

    Recently biologists have been interested in a tide-associated periodic behavior displayed by the diatom Hantzschia virgata, a microscopic golden-brown alga that inhabits that portion of a shoreline washed by tides (the intertidal zone). Diatoms of this species, sometimes called "commuter" diatoms, remain burrowed in the sand during high tide, and emerge on the sand surface during the daytime low tide. Just before the sand is inundated by the rising tide, the diatoms burrow again. Some scientists hypothesize that commuter diatoms know that it is low tide because they sense an environmental change, such as an alteration in temperature or a change in pressure caused by tidal movement. However, when diatoms are observed under constant conditions in a laboratory, they still display periodic behavior, continuing to burrow on schedule for several weeks. This indicates that commuter diatoms, rather than relying on environmental cues to keep time, possess an internal pacemaker or biological clock that enables them to anticipate periodic changes in the environment. A commuter diatom has an unusually accurate biological clock, a consequence of the unrelenting environmental pressures to which it is subjected; any diatoms that do not burrow before the tide arrives are washed away.

    This is not to suggest that the period of this biological clock is immutably fixed. Biologists have concluded that even though a diatom does not rely on the environment to keep time, environmental factors—including changes in the tide's hydrostatic pressure, salinity, mechanical agitation, and temperature—can alter the period of its biological clock according to changes in the tidal cycle. In short, the relation between an organism's biological clock and its environment is similar to that between a wristwatch and its owner: the owner cannot make the watch run faster or slower, but can reset the hands. However, this relation is complicated in intertidal dwellers such as commuter diatoms by the fact that these organisms are exposed to the solar-day cycle as well as to the tidal cycle, and sometimes display both solar-day and tidal periods in a single behavior. Commuter diatoms, for example, emerge only during those low tides that occur during the day.